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Welcome to the High Throughput

 
Crystallization Laboratory

 

 

 


This resource is open to all the members of the Partnership for Structural Biology (PSB) including EMBL, ESRF, ILL, IBS and UVHCI.

Access to this facility for external users is funded through the  inext logo   
project.



In these pages you will find information and instructions about methods available on the platform.

If you want to use our services please register: User Registration page

 

 
 

 

 

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For additional information please contact us at:

Phone: 0033 (0) 476 207425 


e-mail: htx@embl.fr

 

If you use an old web browser, or Internet Explorer, you may have some visual bugs. In this case, we strongly recommend to you update it, or download and install another web browser (Firefox, Chrome, Opera, Safari...).

Also if you do not have administrator rights on your machine, you can use a portable version of these browsers which does not require installation or specific rights.

 

Latest news

Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors published in Nat. Comm.
The HTX platform now supports random Microseed Matrix Screening. rMMS is a powerful tool to significantly increase the hit rate in early stages of crystallization. Read...
Automated pipelines for compound and fragment screening in Grenoble      The EMBL Grenoble, the ESRF and the iNEXT consortium are jointly offering access to automated pipelines for compound and fragment screening based on the CrystalDirect[1] technology and the MASSIF[2] beamlines. Access to these...

Thanks to the introduction of a new crystallization robot, and the optimization of our protocols, we have managed to reduce the sample volumes needed for crystallization trials !

Less sample required for crystallization trials

 

Dear HTX users,


Thanks to the introduction of a new crystallization robot, and the optimization of our protocols, we have managed to reduce the sample volumes needed for crystallization trials, saving up to 16% of sample if you use more than 3 plates.
The protocol now requires 15µl of sample per plate (and per ratio) for the first 3 plates, and then an additional 10µl is required per supplementary plate. See below the detailed volumes for our standard protocols.

 

Number of plate(s) requested
Protocols 1 2 3 4 5 6
Standard 15 µl 30 µl 45 µl 55 µl 65 µl 75 µl
2 ratios 30 µl 60 µl 90 µl 110 µl 130 µl 150 µl
3 ratios 45 µl 90 µl 135 µl 165 µl 195 µl 225 µl


For any other protocols, please contact the HTX staff to check the volume needed. We also recommend providing a couple of microliters extra to compensate potential imprecision during pipetting (especially if you have not calibrated your pipettes recently).

 

The HTX team