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Welcome to the High Throughput

 
Crystallization Laboratory

 

 

 


This resource is open to all the members of the Partnership for Structural Biology (PSB) including EMBL, ESRF, ILL, IBS and UVHCI.

Access to this facility for external users is funded through the  inext logo   
project.



In these pages you will find information and instructions about methods available on the platform.

If you want to use our services please register: User Registration page

 

 
 

 

 

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For additional information please contact us at:

Phone: 0033 (0) 476 207425 


e-mail: htx@embl.fr

 

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Also if you do not have administrator rights on your machine, you can use a portable version of these browsers which does not require installation or specific rights.

 

Latest news

Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors published in Nat. Comm.
The HTX platform now supports random Microseed Matrix Screening. rMMS is a powerful tool to significantly increase the hit rate in early stages of crystallization. Read...
Automated pipelines for compound and fragment screening in Grenoble      The EMBL Grenoble, the ESRF and the iNEXT consortium are jointly offering access to automated pipelines for compound and fragment screening based on the CrystalDirect[1] technology and the MASSIF[2] beamlines. Access to these...

A new set of standard crystallization screens is available at the HTX lab !

New presentation of crystallization screens

 

Dear HTX users,

We have recently changed the way of presenting the different crystallization screens available at the facility. The HTX lab currently offers 35 96-well screens totaling 3360 crystallization conditions. The full list of screens is available under the information tab in the CRIMS web page, while the 10 most commonly used screens are directly available through the crystallization request page.

We have introduced a new selection of 10 screens directly available from the crystallization request page. For this we have taken in account several factors: First, the analysis of the hit tracking results from 2006 to 2014 has allowed us to find out the most successful screens. Secondly, we have taken into account the chemical redundancy among screens and coverage of the crystallization space. The combination of those three parameters has led to a new set of 6 plates: Classics Suite (former plate_1), JCSG, PACT, PEGs, Salt Grid (former plate_4) and Wizard. Furthermore, we offer 4 additional screens Index, MemGold, Morpheus and SaltRX which complement the six standards plates.

  • Index is the former plate_6, that has been removed from standard screenings since it shares 33 conditions with JCSG.
  • MemGold addresses the diversity of membrane proteins studied, both alpha- and beta - types.
  • Morpheus covers a range of pH, precipitants, PEGs, salt, additives and 49 low molecular weight ligands. This screen aims to unlock novel chemical space previously inaccessible using conventional screens.  
  • SaltRX is designed to screen salts of varying concentration and pH.

Should you have any question about the new set of screens, the HTX staff stay at your disposal.

 

The HTX team