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Screenings

 

A link to the full formulation of a screen is available by clicking on its name.

 

Set of 6 Standard Crystallization screens

This set has been designed by the HTX staff for initial crystallization screening.

Its selection is based on analysis of 9 years of crystallization service, and takes into account chemical redundancy among screens and coverage of the crystallization space.

Primary set for initial screening
Plate Description Supplier
Classics Suite Most popular chemicals in protein crystallography with Conditions based on the work by Jancarik and Kim (1). Former plate_1_qiagen Qiagen/Nextal
JCSG+

Optimized sparse matrix screen of classic and modern conditions analyzed by the Joint Centre for Structural Genomics  (2)

Molecular Dimensions
PACT premier PEGs/ions and PEGs/pHs combinations for systematic analysis of cations and anions at defined pHs (2) Molecular Dimensions
PEGs-I Low concentrated salts against PEG 3350 (PEG-ion) plus PEGs of a wide range of molecular weight (200 up to 20,000) at 4 pH Qiagen/Nextal
Salt Grid Made of QuickScreen, Grid screens Ammonium Sulfate, Grid screens Sodium Malonate from Hampton and a home-made grid screen Sodium Formate. Primary or secondary, salt, polymer, organic and pH grid. Former plate_4_hampton Hampton Research - HTX made
Wizard I&II Random sparse matrix for the crystallization of biological macromolecules Rigaku Reagents

 

 

Additional screens 

These additional screens have been selected in order to provide complementary conditions to the initial set of screens.

Additional plates for initial screening
Plate Description Supplier
Index Former plate_6_hampton. Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules. Hampton Research
Morpheus Crystallization screen covering a range of pH, precipitants, PEGs, salt additives and 49 low molecular weight ligands (3). All the conditions are cryo-protected. This screens aims to unlock novel chemical space previously inaccessible using conventional screens. Molecular dimensions
SaltRX  Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules. Hampton Research
Natrix HT Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes Hampton Research
The Nucleix Conditions suited for protein-nucleic acid complexes Qiagen/Nextal
The Midas plus Crystallization screen based on alternative polymeric precipitants (6) including PPGBA, ideal for protein, protein/protein or protein/nucleic acid complexes and sensitive macromolecular complexes. Molecular Dimensions

 

Membrane protein screens 

These screens are dedicated to membrane proteins.

Plate Description Supplier
MemGold  It is based on crystallization conditions data mined from the Protein Data Bank and it addresses the diversity of membrane proteins studied, both alpha- and beta - types. It covers a range of pH, PEGs and salt additives. Molecular dimensions
MemGold 2  Most recent alpha-helical membrane proteins crystallization conditions, particularly suited for prokaryotic and eukaryotic alpha-helical membrane proteins Molecular Dimensions
MemMeso  Semi-systematic screening covering a range of pH, precipitants (PEGs) and salt. Conditions data mined from current reported GPCR crystal structures. Molecular Dimensions
MemStart & MemSys Optimal starting point for screening and optimization of alpha-helical membrane proteins, based on the reagents typically used in the laboratory of Prof. S. Iwata Molecular Dimensions
MemChannel

MemChannel uniquely targets ion channel proteins. Includes the most common ion channel crystallization conditions to maximize your chances of success.

Molecular Dimensions
MemTrans

MemTrans uniquely targets transporter proteins. Includes the most common transporter protein crystallization conditions to maximize your chances of success

Molecular Dimensions
Wizard cubic LCP

The Wizard Cubic™ LCP Screen random sparse matrix is designed for the crystallization of biological macromolecules that are embedded within a lipidic cubic phase (LCP) host matrix.

Rigaku/Molecular Dimensions

 

Additive screens

The following screens are used for optimization of an initial crystallization hit.

They are not available through the crystallization request form. To request them for your crystallization trial, please use the "comment" field of the request form.

Please, note that you have to provide the base solution (15ml) to be optimized.

 

Screen Description Supplier
The Opti-Salts Library of 96 salt additives at different concentrations and pHs Qiagen/Nextal
Additive Screen Library of 96 small molecules affecting sample-sample & sample-solvent interactions to improve crystals or alter sample solubility Hampton Research

 

 

Non-standard screens

More crystallization screens are available. They can be requested for specific needs (nucleic acids, membrane protein...) or for a broader crystallization space coverage. Their stock depends on the demand. Therefore, if you require them, please make your request or contact the HTX staff at least one week in advance.

The following screens are not available through the crystallization request form. To request them for your crystallization trial, please use the "comment" field of the request form. 

 

Screen Description Supplier
PEGRx Large range of MW polymers (200-20,000) with unique buffers from pH 3.5 to pH 9.0 in a medium ionic strength environment including additives, salt, volatile organics and polyols. Hampton Research
The ProPlex Best conditions for protein-protein complexes. Helps to maintain protein-protein interaction by reducing solubility and promoting stability of complexes based on the work of Radaev and Sun (4) and the PCCD (8). (Now replacing the ProComplex screen from Qiagen) Molecular Dimensions
The Helix Crystallize a diverse range of nucleic acids, oligonucleotides, triplexes, quadruplexes, pseudoknots, i-motifs, and large molecular weight nucleic acids Molecular Dimensions
JBScreen Classic 1 PEG-based screen designed for efficient and flexible screening for proteins, peptides, nucleic acids, macromolecular complexes and water-soluble small molecules from data mining of several thousands of crystallized proteins. Jena Bioscience
JBScreen Classic 2 Ammonium sulfate-, MPD-, alcohol- and salt-based screen designed for efficient and flexible screening for proteins, peptides, nucleic acids, macromolecular complexes and water-soluble small molecules from data mining of several thousands of crystallized proteins. Jena Bioscience
The PEGS II Polyethylene glycol of different molecular weight Qiagen/Nextal
The MPD MPD in combination with salts and various pH Qiagen/Nextal
The MbClass I Most successful conditions from literature, specific for membrane proteins. Qiagen/Nextal
The MbClass II Most successful conditions from literature, specific for membrane proteins. Qiagen/Nextal
The Anions Grid screen containing 2 concentrations of salts and 4 different pH values Qiagen/Nextal
The Cations Grid screen containing 2 concentrations of salts and 4 different pH values Qiagen/Nextal
The ComPAS Rapid analysis of polymers, alcohols, and salts. Most successful crystallization conditions from the BMCD (5) Qiagen/Nextal
The JCSG Core I Optimized suites based on over 500,000 crystallization trials performed at the JCSG (7) Qiagen/Nextal
The JCSG Core II Optimized suites based on over 500,000 crystallization trials performed at the JCSG (7) Qiagen/Nextal
The JCSG Core III Optimized suites based on over 500,000 crystallization trials performed at the JCSG (7) Qiagen/Nextal
The JCSG Core IV Optimized suites based on over 500,000 crystallization trials performed at the JCSG (7) Qiagen/Nextal
The Classics II Sparse matrix and ionic sampling screening strategies Qiagen/Nextal
Crystal Screen Crystal Screen I and II. Equivalent to Classics Suite Hampton research
The Cryos Conditions based on the work by Jancarik and Kim (1) with glycerol as cryoprotectant Qiagen/Nextal
Cubic phase I Designed for lipidic cubic phase experiments for membrane protein crystallization. Systematic screening of salt concentration versus pH. Qiagen/Nextal

 

1. Jancarik, J., and Kim, S-H. (1991) Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Cryst. 24, 411. 

2. Newman et al. (2005). Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Cryst. D. 61, 1426-1431

3. Gorrec, F. (2009) The MORPHEUS protein crystallization screen. J. Appl. Cryst. 42, 1035–1042.

4. Radaev, S., and Sun, P. (2002) Crystallization of protein-protein complexes, J. Appl. Cryst. 35, 674.

5. Gilligand, G.l., Tung, M., Blakeslee, D.M. and Ladner, J. (1994) The Biological Macromolecule Crystallization Database, Version 3.0: New features, Data, and the NASA Archive for Protein Crystal Growth Data. Acta Crystallogr. D. 50, 408-413.

6. Grimm, C., Chari, A., Reuter, K. & Fischer, U. (2010) A crystallization screen based on alternative polymeric precipitants. Acta Cryst. D. 66, 685-697.

7. P Lesley, S.A., and Wilson, I.A. (2005) Protein production and crystallization at the joint center for structural genomics. J. Struct. Funct. Genomics. 6, 71.

8. Radaev, S., Li, S., and Sun, P. (2006) A survey of protein-protein complex crystallization, J. Appl. Cryst. D. 62, 605-612.