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Welcome to the High Throughput

 
Crystallization Laboratory

 

 

 


This resource is open to all the members of the Partnership for Structural Biology (PSB) including EMBL, ESRF, ILL, IBS and UVHCI.

Access to this facility for external users is funded through the  inext logo   
project.



In these pages you will find information and instructions about methods available on the platform.

If you want to use our services please register: User Registration page

 

 
 

 

 

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For additional information please contact us at:

Phone: 0033 (0) 476 207425 


e-mail: htx@embl.fr

 

If you use an old web browser, or Internet Explorer, you may have some visual bugs. In this case, we strongly recommend to you update it, or download and install another web browser (Firefox, Chrome, Opera, Safari...).

Also if you do not have administrator rights on your machine, you can use a portable version of these browsers which does not require installation or specific rights.

 

Latest news

Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors published in Nat. Comm.
The HTX platform now supports random Microseed Matrix Screning. rMMS is a powerful tool to significantly increase the hit rate in early stages of crystallization.
Automated pipelines for compound and fragment screening in Grenoble      The EMBL Grenoble, the ESRF and the iNEXT consortium are jointly offering access to automated pipelines for compound and fragment screening based on the CrystalDirect[1] technology and the MASSIF[2] beamlines. Access to these...

Under BioStruct-X scientists working in publicly funded laboratories of EU member states can submit samples for crystallization to the HTX Lab.
Applications are evaluated by a scientific panel and results are communicated within 48 hours.
Next application deadline is September 15th  -  Apply here

Access to the HTX lab for External Users

 

 

BioStruct-X is a project funded by the Seventh Framework Programme (FP7) of the European Commission that establishes a state‐of‐the‐art coordinated and multi‐site infrastructure to support access for established and emerging key methods in structural biology. Eleven facilities from across Europe provide 44 installations for applications in small angle X‐ray scattering (SAXS), macromolecular X‐ray crystallography (MX) biological X ray imaging (XI) protein production and high throughput crystallisation (PP & HTX).

The aim of BioStruct-X is to provide a consolidated platform that brings together all relevant methods in structural biology – creating a single gateway to Europe’s leading European synchrotron facilities and associated infrastructures, ultimately to the benefit of the overall user community.

BioStruct-X cooperates with the ESRFI project Instruct (Integrated Structural Biology Infrastructure for Europe) in aiming to provide an integrated and coordinated technology platform to all relevant methods in structural biology.

For any further questions and inquiries, please mail to: This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

Next application deadline: 15 September 2015

 

To apply for access please fill the form on biostruct logo web site

In order to use our service, select SP (single proposal) while filling the application form. The applicant has to choose the number of HTX units to be granted. One HTX unit corresponds to one 96-well crystallization plate (one to three samples can be set up per plates). Therefore, we recommend to request for 50 HTX units.

 

Mode of Operation

At the HTX lab, the user designs the experiments through our dedicated web interface. Details on the methods and possible experimental parameters are available through our web portal, however specific and advice can be requested from the HTX lab staff at This email address is being protected from spambots. You need JavaScript enabled to view it..">This email address is being protected from spambots. You need JavaScript enabled to view it.. Every individual crystallization experiment is submitted to a quality control process. After the crystallization experiments are set up the user receives an experimental report including data on quality control. Results are available in real-time through our web-based Crystallization Information Management System (CRIMS), which is synchronized with the ESRF sample changer and data collection system ISPyB (this requires the use of SPINE sample loops with 2D-data matrix). Plates are made available to users upon requests, (typically during their next visit to the ESRF beam lines). If necessary, lenses and liquid nitrogen will be made available for crystal mounting and freezing (but no sample loops).

Currently, we are also offering remote access to automated crystal harvesting and cryo-cooling (in collaboration with the Instrumentation Team, EMBL Grenoble) as well as in situ x-ray diffraction experiments (in collaboration with BM14, ESRF), through our own CrystalDirect technology : Acta Cryst. (2012). D68, 1393-1399.

 

Support

The laboratory staff is available for any specific request and can advice users on the best choices for their projects. In addition to the standard procedures, a number of alternative protocols are available to support samples with specific requirements (low stability, detergents etc,). Support for the design of optimization experiments is also available. You can contact the laboratory staff at This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

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