Log in

Login to your account

Username
Password *

Microcapillary Protein Crystallization System (MPCS PlugMaker) 

 

 

 

A Microcapillary Protein Crystallization System (MPCS) is available at the HTX lab. This system is designed to generate diffraction-ready crystals through the use of a specially designed microfluidic chip. Click here if you want to see how it works.

 

The MPCS Plugmaker allows to perform about 200 microbatch experiments with only 5 µL of protein and is specially well suited for the rapid optimization of crystallization hits identified through standard vapour diffusion screening. The system can produce crystals with dimensions of up to several hundred microns that can be recovered with a standard loop and used for X-ray data collection.

Practical Information:

            Each chip can hold two independent refinement experiments. They are available at the This email address is being protected from spambots. You need JavaScript enabled to view it.

            You will need 5 µl of protein per refinement experiment and 5 µL of precipitant and buffer solutions.

            Basic refinement programs and training are provided by the This email address is being protected from spambots. You need JavaScript enabled to view it.


If you want to use the system please contact us at This email address is being protected from spambots. You need JavaScript enabled to view it.

If you what to have additional information on the MPCS plugmaker please click here or look at the references below

References

Gerdts et al. Acta Cryst. (2008). D64, 1116.

Gerdts et al. J. Appl. Cryst. (2010). 43, 1078.

Christensen et al. Acta Cryst. (2011). F67, 1022–1026