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Welcome to the High Throughput

 
Crystallization Laboratory

 

 

 


This resource is open to all the members of the Partnership for Structural Biology (PSB) including EMBL, ESRF, ILL, IBS.

Access to this facility for external users is funded through the  inext logo   
project.



In these pages you will find information and instructions about methods available on the platform.

If you want to use our services please register: User Registration page

 

 
 

 

 

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For additional information please contact us at:

Phone: 0033 (0) 476 207425 


e-mail: htx@embl.fr

 

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Latest news

The symposium "Macromolecules in Action" will be held at the European Photon and Neutron (EPN) science campus in Grenoble, France, from 4-5 July 2019.
Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors published in Nat. Comm.
Automated pipelines for compound and fragment screening in Grenoble      The EMBL Grenoble, the ESRF and the iNEXT consortium are jointly offering access to automated pipelines for compound and fragment screening based on the CrystalDirect[1] technology and the MASSIF[2] beamlines. Access to these...

Under P3 (P-cube) scientists working in publicly funded laboratories of EU member states can submit samples for crystallization to the HTX Lab.
Applications are evaluated by a scientific panel and results are communicated within 48 hours.

Application form

 P3 is a transnational access initiative within the European Framework Program 7 which provides free access to advanced infrastructures and core facilities. Under P3 scientists working in publicly funded laboratories of EU member states can submit samples for fully automated crystallization to the HTX lab in Grenoble.

To benefit form this program applications should be submitted using a web form. Applications will be evaluated by an independent Transnational Access Board and results communicated within 48 hrs. If the application is accepted, researches can send samples related to this project for crystallization to the HTX lab in Grenoble up to four times (a maximum of three samples each time). Beyond this re-application will be necessary. It is possible to apply for more than one project.

The crystallisation facility at EMBL Grenoble Outstation is one of the key platforms of the Partnership for Structural Biology (PSB) and is located next to the European Synchrotron Radiation Facility (ESRF) and the Institute Laue Langevin (ILL). This facility offers completely automated crystallization screening in hanging and sitting drop set up at two temperatures (20°C and 5°C). The facility consists of a high-volume liquid handling station for the production of screens, a Cartesian nanovolume crystallization robot and three automated imaging systems, two operating at 20°C and one at 5°C. Results together with all experimental parameters are made available to users in real time through our CRystallization Information Management System (CRIMS), which includes fast and convenient web user interfaces providing advanced tools like Drop Scoring, Drop history, Navigation through project targets and samples and project summaries among others. CRIMS is synchronized with the ESRF automated sample changers and data collection software through ISPyB. Crystallization plates containing crystals are made available to users upon requests (typically during their next synchrotron visit) and facilities for crystal mounting and freezing are provided. In the six years of operation this facility has provided crystallization screening to more than 300 scientists.

To apply for access please fill this form : Application form

 

Mode of Operation

At the HTX lab the user designs the experiments through our dedicated web interface. Details on the methods and possible experimental parameters are available through our web portal, however specific and advice can be requested from the HTX lab staff at This email address is being protected from spambots. You need JavaScript enabled to view it.. Every individual crystallization experiment is submitted to a quality control process. After the crystallization experiments are set up the user receives an experimental report including data on quality control. Results are available in real-time though our web-based Crystallization Information Management System (CRIMS), which is synchronized with the ESRF sample changer and data collection system ISPyB (this requires the use of SPINE sample loops with 2D-data matrix). Plates are made available to users upon requests, (typically during their next visit to the ESRF beam lines). If necessary, lenses and liquid nitrogen will be made available for crystal mounting and freezing (but no sample loops).

Support

The laboratory staff is available for any specific request and can advice users on the best choices for their projects. In addition to the standard procedures, a number of alternative protocols are available to support samples with specific requirements (low stability, detergents etc,). Support for the design of optimisation experiments is also available. You can contact the laboratory staff at This email address is being protected from spambots. You need JavaScript enabled to view it.

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